Protocols

This site summarizes Standard Operating Procedures (SOPs) developed and employed by EuroKUP members for biological specimen (urine-tissue) collection. These protocols have been widely employed for proteomics analysis and are therefore recommended to be used in the clinical setting

  • Collection of urine samples for peptidome (e.g. LC-MS or CE-MS) analysis
    To achieve standardization in the clinical setting and facilitate data comparability, a detailed protocol for urine collection has been prepared and can be downloaded here
  • Rat and mouse kidneys preparation
    This protocol for rat and mouse kidneys preparation was used in the Rotterdam training school. It can be downloaded here
  • Collection of urine samples for proteome analysis
    A detailed protocol for urine collection employed in Prof Roz Bank's laboratory is described in the attached file. It can be downloaded here
  • Standard Protocol for Urine Collection
    Protocol for Urine Collection as recommended by the Human Kidney and Urine Proteome Project, HKUPP (www.hkupp.org), and the European Urine and Kidney Proteomics, EuroKUP, Initiatives. It can be downloaded here
  • Laser capture microdissection of formaline fixed paraffin embedded tissue for proteome analysis
    Protocol for Laser capture microdissection of formaline fixed paraffin embedded tissue for proteome analysis. It can be downloaded here

Our protocols are freely available here and in the Molecular Methods Database MolMeth.

About the author

Martin Norling's picture

Full name
Martin Norling

Affiliation
The Linnaeus Centre for Bioinformatics Uppsala University / SLU

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